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Image Search Results
Journal: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
Article Title: High-throughput quantitative real-time polymerase chain reaction array for absolute and relative quantification of rhesus macaque types I, II, and III interferon and their subtypes.
doi: 10.1089/jir.2012.0015
Figure Lengend Snippet: FIG. 4. IFN-a2 protein concentration in the PBMC cultures. Protein levels were quantified by ELISA in the culture su- pernatants of 3 rhesus macaque PBMCs stimulated with LPS (10 mg/mL), poly I:C (50 mg/mL), or imiquimod (10 mg/mL) for 25 h (mean of 3 replicates – SD).
Article Snippet: PCRs were conducted in a 7900HT Real-Time PCR System (Applied Biosystems) according to the following conditions: 50.0 C for 2 min, followed by 95.0 C for 10 min, 40 cycles of 95.0 C for 15 s, and 59.5 C for 1 min. ELISA for IFN-a protein The culture supernatants of TLR-stimulated rhesus PBMCs were harvested after 25h, and the concentration of IFN-a2 protein was measured in triplicate with the
Techniques: Protein Concentration, Enzyme-linked Immunosorbent Assay